MTOR (phospho-T2446) polyclonal antibody

Product Specifications

Background

MTOR, or FKBP12-rapamycin associated protein (FRAP), is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 245-kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Centrosomal protein of 70 kDa; Cep70; p10-binding protein; CEP70; BITE

Swiss Prot

P42345

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 246-306 of Human CEP70.

Conjugation

Unconjugated

Related Pathways

Phosphoinosotode Signaling, PI3K AKT signaling, Hypoxia Signaling, Warburg Effect, Insulin &Glucose Signaling, Glutamine Metabolism, MTOR signaling, ErbB HER Signaling

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 289 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-mTOR (T2446) pAb detects endogenous levels of mTOR protein only when phosphorylated at Thr2446.

Applications Notes

Western blot (WB) analysis of p-mTOR (T2446) pAb at 1:500 dilution Lane1:SK-OVCAR3 whole cell lysate (40µg) Lane2:HEK293T whole cell lysate (40µg) Lane3:CT-26 whole cell lysate (40µg) Lane4:C6 whole cell lysate (40µg)