Cdk5 (phospho-Y15) polyclonal antibody

Product Specifications

Background

Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks) . Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression. Another family of proteins, Cdk inhibitors, also plays a role in regulating cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1–3, PITALRE and PITSLRE. Cdk5 is thought to be involved in the G1/S transition of the cell cycle and is highly expressed in mature neurons. Activity of Cdk5 increases significantly during neuronal differentiation. Cdk5 has been postulated to be a neurofilament or Tau protein kinase, based on its ability to phosphorylate these proteins in vitro.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Cyclin-dependent-like kinase 5; Cell division protein kinase 5; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit; CDK5; CDKN5

Swiss Prot

Q00535

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Cdk5 around the phosphorylation site of Tyrosine15.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 33 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Cdk5 (phospho-Y15) polyclonal antibody detects endogenous levels of Cdk5 protein only when phosphorylated at Tyr15.

Applications Notes

Western blot (WB) analysis of Cdk5 (phospho-Y15) polyclonal antibody at 1:500 dilution Lane1:U-87MG whole cell lysate (40ug) Lane2:A2780 whole cell lysate (40ug) Lane3:SK-OVCAR3 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug)