Cdk5 (phospho-Y15) polyclonal antibody
Product Specifications
Background
Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks) . Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression. Another family of proteins, Cdk inhibitors, also plays a role in regulating cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1–3, PITALRE and PITSLRE. Cdk5 is thought to be involved in the G1/S transition of the cell cycle and is highly expressed in mature neurons. Activity of Cdk5 increases significantly during neuronal differentiation. Cdk5 has been postulated to be a neurofilament or Tau protein kinase, based on its ability to phosphorylate these proteins in vitro.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Cyclin-dependent-like kinase 5; Cell division protein kinase 5; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit; CDK5; CDKN5
Swiss Prot
Q00535
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Cdk5 around the phosphorylation site of Tyrosine15.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 33 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Cdk5 (phospho-Y15) polyclonal antibody detects endogenous levels of Cdk5 protein only when phosphorylated at Tyr15.
Applications Notes
Western blot (WB) analysis of Cdk5 (phospho-Y15) polyclonal antibody at 1:500 dilution Lane1:U-87MG whole cell lysate (40ug) Lane2:A2780 whole cell lysate (40ug) Lane3:SK-OVCAR3 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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