KIF20A (phospho-S528) polyclonal antibody

Product Specifications

Background

KIF20A is a member of the kinesin-like protein family and has 1 kinesin motor domain. It interacts with guanosine triphosphate (GTP) -bound forms of RAB6A and RAB6B and may act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. KIF20A has a microtubule plus end-directed motility.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Kinesin-like protein KIF20A; GG10_2; Mitotic kinesin-like protein 2; MKlp2; Rab6-interacting kinesin-like protein; Rabkinesin-6; KIF20A; MKLP2; RAB6KIFL

Swiss Prot

O95235

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human KIF20A around the phosphorylation site of Ser125.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 125 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

KIF20A (phospho-S528) polyclonal antibody detects endogenous levels of KIF20A protein when phosphorylated at Ser528.

Applications Notes

Western blot (WB) analysis of p-KIF20A (S528) pAb at 1:500 dilution Lane1:PC12 whole cell lysate (40µg) Lane2:The Testis tissue lysate of Mouse (40µg) Lane3:SK-OVCAR3 whole cell lysate (40µg) Lane4:Myla2059 whole cell lysate (40µg)