Smad2/3 (phospho-T8) polyclonal antibody
Product Specifications
Background
Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of GFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD2; MADH2; MADR2
Swiss Prot
P84022/Q15796
Reactivity
Human, Mouse
Immunogen
Synthetic phosphopeptide derived from human Smad2/3 around the phosphorylation site of Threonine 8.
Conjugation
Unconjugated
Related Pathways
Tumor Angiogenesis, Cell Cycle Control G1 S Checkpoint, Contribution of Soluble Factors to EMT, ESC Pluripotency and Differentiation Signaling Pathway, hippo Signaling, hippo Signaling
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 55, 60 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-Smad2/3 (T8) polyclonal antibody detects endogenous levels of Smad2/3 protein only when phosphorylated at Thr8.
Applications Notes
Western blot (WB) analysis of p-Smad2/3 (T8) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (40µg) Lane2:Hela whole cell lysate (40µg) Lane3:The Lung tissue lysate of Mouse (40µg) Lane4:SGC7901 whole cell lysate (40µg) Lane5:H9C2 whole cell lysate (40µg)
Host or Source
Rabbit
Available Sizes
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