DAPK3 (phospho-T265) polyclonal antibody

Product Specifications

Background

Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certainserine/threonine protein kinases, such as ASK1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase. ZIP kinase contains an N terminal kinase domain and a C terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREBfamily. ZIP kinase has high sequence homology to DAP kinase (death associated protein kinase), which is a mediator of apoptosis induced by gamma interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

ZIPK; ZIP kinase; ZIP-kinase; ZIP; Dlk; Death associated protein kinase 3; Death associated kinase 3; Dapk3; Dapk 3; DAP like kinase; DAP kinase 3

Swiss Prot

O43293

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human DAPK3 around the phosphorylation site of Thr265.

Conjugation

Unconjugated

Related Pathways

Epigenetic Writers and Erasers of Histone H3

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 52 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

DAPK3 (phospho-T265) polyclonal antibody detects endogenous levels of DAPK3 protein when phosphorylated at Thr265.

Applications Notes

Western blot (WB) analysis of p-DAPK3 (T265) pAb at 1:500 dilution Lane1:Hela whole cell lysate (40µg) Lane2:SK-OVCAR3 whole cell lysate (40µg) Lane3:SGC7901 whole cell lysate (40µg) Lane4:3T3-L1 whole cell lysate (40µg) Lane5:PC12 whole cell lysate (40µg)