Cytokeratin 18 (phospho-S52) polyclonal antibody
Product Specifications
Background
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments. Keratin isoforms demonstrate tissue and differentiation-specific profiles that make them useful as research biomarkers. Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Keratin, type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18; KRT18; CYK18; PIG46
Swiss Prot
P05783
Reactivity
Human
Immunogen
Synthetic phosphopeptide derived from human Cytokeratin 18 around the phosphorylation site of Serine 52
Conjugation
Unconjugated
Related Pathways
Contribution of Soluble Factors to EMT
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 46 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-Cytokeratin 18 (S52) polyclonal antibody detects endogenous levels of Cytokeratin 18 protein only when phosphorylated at Ser52.
Applications Notes
Western blot (WB) analysis of p-Cytokeratin 18 (S52) polyclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate (40µg) Lane2:L02 whole cell lysate (40µg) Lane3:A549 whole cell lysate (40µg)
Host or Source
Rabbit
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