PKC ε (phospho-S729) polyclonal antibody

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (α, βI, βII, γ, ∂, é, Ω, h, †, λ/î, µ and n) . Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC ∂ and é are independent of Ca++. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of PKC ε (phospho-S729) polyclonal antibody at 1:500 dilution Lane1:HEK293 whole cell lysate (40µg) Lane2:U-87MG whole cell lysate (40µg) Lane3:SGC7901 whole cell lysate (40µg) Lane4:SGC7901 treated with PMA (50ng/ml) for 5 minutes then repair for 10 minutes whole cell lysate (40µg) Lane5:SGC7901 treated with PMA (50ng/ml) for 5 minutes then repair for 30 minutes whole cell lysate (40µg)