KOR-1 (phospho-S369) polyclonal antibody

Product Specifications

Background

Endogenous opioid peptides and opiates, like morphine, transmit their pharmacological effects through membrane bound opioid receptors. Pharmacological studies and molecular cloning have led to the identification of three different types of opioid receptor, mu-type, delta-type and kappa-type, also designated MOR-1, DOR-1 and KOR-1, respectively. MOR-1 is a receptor for beta-endorphin, DOR-1 is a receptor for enkephalins, and KOR-1 is a receptor for dynorphins. The three opioid receptor types are highly homologous and belong to the superfamily of G-protein-coupled receptors. Opioid receptors have been shown to modulate a range of brain functions, including instinctive behavior and emotions. This regulation is thought to involve the inhibition of neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Kappa-type opioid receptor; K-OR-1; KOR-1; OPRK1; OPRK

Swiss Prot

P41145

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human KOR-1 around the phosphorylation site of Ser369.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 42 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

KOR-1 (phospho-S369) polyclonal antibody detects endogenous levels of KOR-1 protein when phosphorylated at Ser369.

Applications Notes

Western blot (WB) analysis of p-KOR-1 (S369) pAb at 1:500 dilution Lane1:U-87MG whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:The Brain tissue lysate of Mouse (40ug) Lane5:The Brain tissue lysate of Rat (40ug)