Smad1 (phospho-S465) polyclonal antibody
Product Specifications
Background
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Mothers against decapentaplegic homolog 1; MAD homolog 1; Mothers against DPP homolog 1; JV4-1; Mad-related protein 1; SMAD family member 1; SMAD 1; Smad1; hSMAD1; Transforming growth factor-beta-signaling protein 1; BSP-1; SMAD1; BSP1; MADH1; MADR1
Swiss Prot
Q15797
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Smad1 around the phosphorylation site of Serine 465.
Conjugation
Unconjugated
Related Pathways
Contribution of Soluble Factors to EMT, ESC Pluripotency and Differentiation Signaling Pathway, hippo Signaling, TGFβ Smad signaling
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 60 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-Smad1 (S465) polyclonal antibody detects endogenous levels of Smad1 protein only when phosphorylated at Ser465.
Applications Notes
Western blot (WB) analysis of p-Smad1 (S465) pAb at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:A549 treated with UV for 5 minutes then repair for 1 hour whole cell lysate (40ug) Lane3:A549 treated with UV for 5 minutes then repair for 3 hours whole cell lysate (40ug) Lane4:A549 treated with UV for 5 minutes then repair for 6 hours whole cell lysate (40ug) Lane5:A549 treated with UV for 5 minutes then repair for 24 hours whole cell lysate (40ug) Lane6:The Uterus tissue lysate of Mouse (40ug) Lane7:The Uterus tissue lysate of Rat (40ug)
Host or Source
Rabbit
Available Sizes
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