Stat5 (phospho-S726/731) polyclonal antibody
Product Specifications
Background
Stat 5a protein is a transcription factor activated by hormone and cytokines. Two highly related, but distinct Stat 5 genes (Stat 5a and Stat 5b) were identified in mouse. The amino acid sequences of Stat 5a and Stat 5b show 96% sequence similarity, and both proteins are co expressed in most tissues of both virgin and lactating mice. However, differential accumulation of Stat 5a and Stat 5b mRNA has been reported for both muscle and mammary tissue. Stat 5a is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Signal transducer and activator of transcription 5A; STAT5A; STAT5; Signal transducer and activator of transcription 5B; STAT5B
Swiss Prot
P42229/P51692
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human stat5a around the phosphorylation site of S726.
Conjugation
Unconjugated
Related Pathways
Immune Cell Signaling, Jak Stat : IL-6 Receptor Signaling, T cell receptor signaling
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 90 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Stat5 (phospho-S726/731) polyclonal antibody detects endogenous levels of Stat5a protein only when phosphorylated at Ser726 and/or Stat5b protein only when phosphorylated at Ser731.
Applications Notes
Western blot (WB) analysis of p-Stat5 (S726/731) pAb at 1:500 dilution Lane1:L02 whole cell lysate (30ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug) Lane5:PC12 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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