Stat5 (phospho-S726/731) polyclonal antibody

Product Specifications

Background

Stat 5a protein is a transcription factor activated by hormone and cytokines. Two highly related, but distinct Stat 5 genes (Stat 5a and Stat 5b) were identified in mouse. The amino acid sequences of Stat 5a and Stat 5b show 96% sequence similarity, and both proteins are co expressed in most tissues of both virgin and lactating mice. However, differential accumulation of Stat 5a and Stat 5b mRNA has been reported for both muscle and mammary tissue. Stat 5a is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Signal transducer and activator of transcription 5A; STAT5A; STAT5; Signal transducer and activator of transcription 5B; STAT5B

Swiss Prot

P42229/P51692

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human stat5a around the phosphorylation site of S726.

Conjugation

Unconjugated

Related Pathways

Immune Cell Signaling, Jak Stat : IL-6 Receptor Signaling, T cell receptor signaling

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 90 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Stat5 (phospho-S726/731) polyclonal antibody detects endogenous levels of Stat5a protein only when phosphorylated at Ser726 and/or Stat5b protein only when phosphorylated at Ser731.

Applications Notes

Western blot (WB) analysis of p-Stat5 (S726/731) pAb at 1:500 dilution Lane1:L02 whole cell lysate (30ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug) Lane5:PC12 whole cell lysate (40ug)