PKC α (phospho-T505) polyclonal antibody

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η and θ) . Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca2+.

Phospholipase Signaling, Protein Kinase C Signaling, Inhibition of Apoptosis, G Protein-coupled Receptors Signaling to MAPK Erk, Mitochondrial Control of Apoptosis

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of p-PKC α (T505) pAb at 1:500 dilution Lane1:Hela whole cell lysate (10ug) Lane2:The Kidney tissue lysate of Mouse (40ug) Lane3:The Heart tissue lysate of Rat (20ug)