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RPS6 (phospho-S240) polyclonal antibody

Product Specifications

Background

Ribosomal protein S6 is a component of the small 40S ribosomeal subunit and belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

40S ribosomal protein S6; Phosphoprotein NP33; RPS6; OK/SW-cl.2

Swiss Prot

P62753

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Ribosomal Protein S6 around the phosphorylation site of Serine 240.

Conjugation

Unconjugated

Related Pathways

PI3K AKT signaling, Immune Cell Signaling, T cell receptor signaling

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 29 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Ribosomal Protein S6 (S240) polyclonal antibody detects endogenous levels of Ribosomal Protein S6 protein only when phosphorylated at Ser240

Applications Notes

Western blot (WB) analysis of RPS6 (phospho-S240) polyclonal antibody at 1:500 dillution Lane1:Hela whole cell lysate (40μg) Lane2:Hela treated with serum starved (24h) whole cell lysate (40μg) Lane3:PC12 whole cell lysate (40μg) Lane4:PC12 treated with serum starved (24h) whole cell lysate (40μg)

Host or Source

Rabbit

Available Sizes

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