RyR2 (phospho-S2808) polyclonal antibody

Product Specifications

Background

Dihydropyridine receptor (DHPR) is a surface membrane protein critical for the excitation-contraction coupling of striated muscle. DHPR and the sarcoplasmic reticulum ryanodine receptor (RyR) are two key components of the intracellular junctions, where depolarization of the surface membrane is converted into the release of Ca2+ from internal stores. The α1-subunit of the DHPR contains a cytoplasmic loop which is thought to be involved in the interactions with RyR. Phosphorylation of the DHPR α1-subunit is also thought to play a role in the functional interaction of DHPR and RyR. Mutation in DHPR α1 results in excitation-contraction uncoupling, leading to muscular dysgenesis, a complete inactivity in developing skeletal muscles. Cells that do not express RyR also lack excitation-contraction coupling and exhibit a severalfold reduction in Ca2+ current density.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Ryanodine receptor 2; RYR-2; RyR2; hRYR-2; Cardiac muscle ryanodine receptor; Cardiac muscle ryanodine receptor-calcium release channel; Type 2 ryanodine receptor; RYR2

Swiss Prot

Q92736

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human RyR2 around the phosphorylation site of Serine 2808.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 565 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-RyR2 (S2808) pAb detects endogenous levels of RyR2 protein only when phosphorylated at Ser2808

Applications Notes

Western blot (WB) analysis of p-RyR2 (S2808) pAb at 1:500 dilution Lane1:PC3 whole cell lysate (40ug) Lane2:K562 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:U-87MG whole cell lysate (40ug)