EIF4B (phospho-S422) polyclonal antibody
Product Specifications
Background
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.It is phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1.phosphorylation enhances the affinity of EIF4B for the EIF3 complex
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Eukaryotic translation initiation factor 4B; eIF-4B; EIF4B
Swiss Prot
P23588
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human eIF4B around the phosphorylation site of Serine 422.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 80 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-eIF4B (S422) polyclonal antibody detects endogenous levels of eIF4B protein only when phosphorylated at Ser422
Applications Notes
Western blot (WB) analysis of p-eIF4B (S422) pAb at 1:500 dilution Lane1:The Brain tissue lysate of Rat (40ug) Lane2:K562 whole cell lysate (40ug) Lane3:A375 whole cell lysate (40ug) Lane4:HCT116 whole cell lysate (40ug)
Host or Source
Rabbit
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