EGFR (phospho-Y992) polyclonal antibody
Product Specifications
Background
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Epidermal growth factor receptor; EC=2.7.10.1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; ERBB; ERBB1; HER1; EGFR
Swiss Prot
P00533
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human EGFR around the phosphorylation site of Tyrosine 992.
Conjugation
Unconjugated
Related Pathways
ErbB HER Signaling
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
Modification
Phosphorylation
Molecular Weight
~ 175 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-EGFR (Y992) polyclonal antibody detects endogenous levels of EGFR protein only when phosphorylated at Tyr992 (removal of the signal peptide) .
Applications Notes
Western blot (WB) analysis of p-EGFR (Y992) ) pAb at 1:500 dilution Lane1:HCC827 whole cell lysate (20ug) Lane2:A549 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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