PRAS40 (phospho-T246) polyclonal antibody
Product Specifications
Background
Akt, also known as protein kinase B, is one of the major downstream targets of the phosphatidylinositol 3-kinase pathway. This protein kinase has been implicated in insulin signaling, stimulation of cellular growth, inhibition of apoptosis and transformation of cells. The proline-rich Akt substrate PRAS40, also designated AKT1S1, becomes phosphorylated by activated Akt on Ser or Thr residues in the motif RXRXX (S/T) . Phosphorylated PRAS40 subsequently binds 14-3-3 in a sequence-specific manner, thereby inducing such changes as alteration of protein subcellular localization and regulation of intrinsic enzymatic activity. Studies also suggest that PRAS40 phosphorylation and its interaction with pAkt and 14-3-3 may play an important role in neuroprotection mediated by NGF in apoptotic neuronal cell death after cerebral ischemia. PRAS40 maps to human chromosome 19q13.33.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate; AKT1S1; PRAS40
Swiss Prot
Q96B36
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human PRAS40 around the phosphorylation site of Threonine 246.
Conjugation
Unconjugated
Related Pathways
PI3K AKT signaling, Translational Contral elF4 and p70 S6 Kinase, Insulin &Glucose Signaling, MTOR signaling
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 40 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-PRAS40 (T246) polyclonal antibody detects endogenous levels of PRAS40 only when phosphorylated at Thr246.
Applications Notes
Western blot (WB) analysis of p-PRAS40 (T246) polyclonal antibody at 1:500 dilution Lane1:LO2 cell lysate Lane2:Raw264.7 cell lysate Lane3:NIH-3T3 cell lysate
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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