AKT1 (phospho-S246) polyclonal antibody

Product Specifications

Background

AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB; RAC; PKBα

Swiss Prot

P31749

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Serine 81.

Conjugation

Unconjugated

Related Pathways

Phosphoinosotode Signaling, PI3K AKT signaling, Protein Kinase C Signaling, Warburg Effect, T cell receptor signaling, Inhibition of Apoptosis, Insulin &Glucose Signaling, Cell Cycle Control G1 S Checkpoint, Adherens Junction signaling, Mitochondrial Control of Apoptosis, MTOR signaling, ErbB HER Signaling

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 60 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-AKT1 (S246) polyclonal antibody detects endogenous levels of AKT1 protein only when phosphorylated at Ser246.This antibody does not cross-react with AKT2 or AKT3.

Applications Notes

Western blot (WB) analysis of AKT1 (phospho-S246) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate Lane2:L02 treated with EGF (0.1ng/ml,5min) whole cell lysate Lane3:L02 treated with EGF (0.1ng/ml,10min) whole cell lysate Lane4:L02 treated with EGF (0.1ng/ml,15min) whole cell lysate Lane5:The Heart tissue lysate of Mouse (40ug) Lane6:The fetal Heart tissue lysate of Rat (40ug)