FoxO1/3/4 (phospho-T24/32) polyclonal antibody

FOXO1 + FOX3 + FOX4 belong to the forkhead family of transcription factors.FOXO1 (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein. The specific function of the gene has not yet been determined.FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia.FOX4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.

Forkhead box protein O4; Fork head domain transcription factor AFX1; AFX; AFX1; MLLT7; FOXO4; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; FOXO1; FKHR; FOXO1A; Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO3; FKHRL1; FOXO3A

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

P-FoxO1/3/4 (T24/32) polyclonal antibody detects endogenous levels of FoxO1 protein only when phosphorylated at Thr24. This antibody also detects endogenous levels of FoxO3 and FoxO4 protein only when phosphorylated at Thr32.

Western blot (WB) analysis of p-FoxO1/3/4 (T24/32) pAb at 1:500 dilution Lane1:HepG2 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:A2780 whole cell lysate (40ug) Lane4:L02 whole cell lysate (40ug)