Tau (phospho-T231) polyclonal antibody

Product Specifications

Background

Tau, also known as MAPT (microtubule-associated protein tau), MAPTL, MTBT1 or TAU, is a 758 amino acid protein that localizes to the cytoplasm, as well as to the cytoskeleton and the cell membrane, and contains four Tau/MAP repeats. Expressed in neuronal tissue and existing as multiple alternatively spliced isoforms, Tau functions to promote microtubule assembly and stability and is thought to be involved in the maintenance of neuronal polarity. Tau may also link microtubules with neural plasma membrane components and, addition to its role in microtubule stability, is also necessary for cytoskeletal plasticity.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Microtubule-associated protein tau; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; MAPT; MAPTL; MTBT1; TAU

Swiss Prot

P10636

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human isoforms Tau-4 around the phosphorylation site of Threonine 231.

Conjugation

Unconjugated

Related Pathways

Neuronal and Glial Cell Marker Atlas, Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer's Disease

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

50 to 80 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Tau (T231) polyclonal antibody detects endogenous levels of Tau only when phosphorylated at Thr548, isoforms Tau-3 and isoforms Tau-4 phosphorylated at Thr231, and other isoforms phosphorylated at the corresponding Site.

Applications Notes

Western blot (WB) analysis of Tau (phospho-T231) polyclonal antibody at 1:500 dillution Lane1:Hela whole cell lysate (40μg) Lane2:Hela treated with EGF (0.1ng/ml, 30min) whole cell lysate (40μg) Lane3:C6 whole cell lysate (40μg) Lane4:C6 treated with EGF (0.1ng/ml, 30min) whole cell lysate (40μg)