What is the catalog number for XRCC5 (phospho-T714) polyclonal antibody?

The catalog number for XRCC5 (phospho-T714) polyclonal antibody is BS4240.

What sizes are available for XRCC5 (phospho-T714) polyclonal antibody?

XRCC5 (phospho-T714) polyclonal antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does XRCC5 (phospho-T714) polyclonal antibody come in?

XRCC5 (phospho-T714) polyclonal antibody is available in 2 different pack sizes.

Is XRCC5 (phospho-T714) polyclonal antibody in stock?

XRCC5 (phospho-T714) polyclonal antibody is currently made to order / not held in immediate stock. Contact us for lead time.

XRCC5 (phospho-T714) polyclonal antibody

Product Specifications

Background

XRCC5 encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V (D) J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V (D) J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

X-ray repair cross-complementing protein 5; 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; Ku80; Ku86; Ku-80; Ku-86; Lupus Ku autoantigen protein p86; Nuclear factor IV; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) ; XRCC5; G22P2

Swiss Prot

P13010

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human XRCC5 around the phosphorylation site of Threonine 714.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 82 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-XRCC5 (T714) polyclonal antibody detects endogenous levels of XRCC5 protein only when phosphorylated at Thr714

Applications Notes

Western blot (WB) analysis of p-XRCC5 (T714) pAb at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:A549 treated with UV for 5 minutes then repair for 1 hour whole cell lysate (40ug) Lane3:A549 treated with UV for 5 minutes then repair for 6 hours whole cell lysate (40ug) Lane4:The Brain tissue lysate of Mouse (40ug) Lane5:The Uterus tissue lysate of Rat (40ug)