IRS-1 (phospho-S1101) polyclonal antibody
Product Specifications
Background
IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4) . IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Insulin receptor substrate 1; IRS-1; IRS1
Swiss Prot
P35568
Reactivity
Human
Immunogen
Synthetic phosphopeptide derived from human IRS-1 around the phosphorylation site of Serine 1101.
Conjugation
Unconjugated
Related Pathways
Translational Contral elF4 and p70 S6 Kinase, Insulin &Glucose Signaling
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 180 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-IRS-1 (S1101) polyclonal antibody detects endogenous levels of IRS-1 protein only when phosphorylated at Ser1101.
Applications Notes
Western blot (WB) analysis of p-IRS-1 (S1101) pAb at 1:500 dilution Lane1:A375 whole cell lysate (40ug) Lane2:Panc1 whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
