HSP90B (phospho-S254) polyclonal antibody

Product Specifications

Background

HSP 90 family members guide the normal folding, intracellular disposition and proteolytic turnover of many key regulators of cell growth, differentiation and survival. HSP 90alpha, also designated HSP90A, HSP 86 and LPS-associated protein 2 (LAP2), is a cytosolic enhancer of inducible nitric-oxide synthase (iNOS), with chaperone activity that is important for the transcriptional activity of p53. HSP 90beta, also designated HSP90B, HSP 84 and HSPC2, is a cytosolic protein that participates in signaling pathways with PKC epsilon to protect cells from external damage, particularly in heat shock-mediated events. GRP 94, also known as tumor rejection antigen 1 (TRA1), ECGP and GP96, localizes to the ER, is highly expressed in BGC-823 human gastric carcinoma cells and is upregulated in human endothelial cells in response to hypoxia by HIF-1.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Heat shock protein HSP 90-beta; HSP 90; Heat shock 84 kDa; HSP 84; HSP84; HSP90AB1; HSP90B; HSPC2; HSPCB

Swiss Prot

P08238

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human HSP90B around the phosphorylation site of Serine 254.

Conjugation

Unconjugated

Related Pathways

Regulation of Actin Dynamics, Hypoxia Signaling

Applications

WB IHC IF

Dilution

WB: 1:1000~1:5000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 90 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

HSP90B (phospho-S254) polyclonal antibody detects endogenous levels of HSP90B protein when phosphorylated at Ser254.

Applications Notes

Western blot (WB) analysis of HSP90B (phospho-S254) pAb at 1:5000 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:The Testis tissue lysate of Mouse (40ug) Lane3:The Testis tissue lysate of Rat (40ug) Lane4:HEK293T whole cell lysate (40ug) Lane5:Hela whole cell lysate (40ug)