GRF-1 (phospho-Y1105) polyclonal antibody
Product Specifications
Background
GRF-1 (glucocorticoid receptor DNA-binding factor 1), also known as p190RhoGAP or simply p190, is a transcriptional regulator which binds to the promoter region of the glucocorticoid receptor gene and represses its expression. By repressing GR expression, GRF-1 acts to down-regulate Rho signaling, thereby mediating both actin cytoskeletal rearrangements and cell cycle events. Through its GAP domain, GRF-1 is thought to affect cytokinesis by regulating Rho activity; a regulation that is controlled by the ubiquination of the GTP binding region and subsequent degradation of GRF-1. Additionally, GRF-1 plays an important role in oligodendrocyte differentiation, a process that is absent in malignant glioma tumors, implicating GRF-1 as a possible tumor suppressor. GRF-1 expression is regulated by glucocorticoids and the expressed protein exists as two isoforms produced by alternative splicing events.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Rho GTPase-activating protein 35; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF-1; Rho GAP p190A; p190-A; ARHGAP35; GRF1; GRLF1; KIAA1722
Swiss Prot
Q9NRY4
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human GRF-1 around the phosphorylation site of Tyrosine 1105.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 172 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-GRF-1 (Y1105) polyclonal antibody detects endogenous levels of GRF-1 protein only when phosphorylated at Tyr1105.
Applications Notes
Western blot (WB) analysis of p-GRF-1 (Y1105) pAb at 1:500 dilution Lane1:The Heart tissue lysate of Rat (40ug) Lane2:K562 whole cell lysate (40ug) Lane3:Jurkat whole cell lysate (40ug) Lane4:The Blood tissue lysate of Mouse (40ug) Lane5:H1792 whole cell lysate (40ug) Lane6:HEK293T whole cell lysate (40ug) Lane7:SP2/0 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
