RUNX1 (phospho-S249) polyclonal antibody

Product Specifications

Background

AML1 (also known as Runx1, CBFA2, and PEBP2αB) is a member of the core binding factor (CBF) family of transcription factors. It is required for normal development of all hematopoietic lineages. AML1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t (8;21) AML1-ETO, t (12;21) TEL-AML, and t (8;21) AML-M2. Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Runt-related transcription factor 1; Acute myeloid leukemia 1 protein; Core-binding factor subunit alpha-2; CBF-alpha-2; Oncogene AML-1; Polyomavirus enhancer-binding protein 2 alpha B subunit; PEA2-alpha B; PEBP2-alpha B; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; RUNX1; AML1; CBFA2

Swiss Prot

Q01196

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human RUNX1 around the phosphorylation site of Serine 249.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 55 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

RUNX1 (phospho-S249) polyclonal antibody detects endogenous levels of RUNX1 protein only when phosphorylated at Ser249.

Applications Notes

Western blot (WB) analysis of p-RUNX1 (S249) pAb at 1:500 dilution Lane1:3T3-L1 whole cell lysate (40ug) Lane2:The Thymus tissue lysate of Rat (40ug) Lane3:K562 whole cell lysate (40ug) Lane4:Myla2059 whole cell lysate (40ug)