Vav1 (phospho-Y174) polyclonal antibody

Product Specifications

Background

The Vav gene was originally identified on the basis of its oncogenic activation during the course of gene transfer assays. The major translational product of the Vav proto-oncogene has been identified as a protein containing an array of structural motifs. Contained within its amino terminus are a helix-loop-helix domain and a leucine zipper motif similar to that of Myc family proteins; deletion of this region of p95Vav causes its oncogenic activation. In addition, p95Vav contains an SH2 domain, which could indicate its role as a substrate for tyrosine kinases. Expression of p95Vav is limited exclusively to cells of hematopoietic origin, including those of the erythroid, lymphoid and myeloid lineages.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Proto-oncogene vav; VAV1; VAV

Swiss Prot

P15498

Reactivity

Human, Rat

Immunogen

Synthetic phosphopeptide derived from human VaV1 around the phosphorylation site of Tyrosine 174.

Conjugation

Unconjugated

Related Pathways

Regulation of Actin Dynamics

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 100 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Vav1 (Y174) polyclonal antibody detects endogenous levels of Vav1 protein when phosphorylated at Tyr174.

Applications Notes

Western blot (WB) analysis of p-Vav1 (Y174) pAb at 1:500 dilution Lane1:THP-1 whole cell lysate (40ug) Lane2:HuT78 whole cell lysate (40ug) Lane3:The Spleen tissue lysate of Mouse (40ug) Lane4:The Spleen tissue lysate of Rat (40ug)