Vav1 (phospho-Y174) polyclonal antibody
Product Specifications
Background
The Vav gene was originally identified on the basis of its oncogenic activation during the course of gene transfer assays. The major translational product of the Vav proto-oncogene has been identified as a protein containing an array of structural motifs. Contained within its amino terminus are a helix-loop-helix domain and a leucine zipper motif similar to that of Myc family proteins; deletion of this region of p95Vav causes its oncogenic activation. In addition, p95Vav contains an SH2 domain, which could indicate its role as a substrate for tyrosine kinases. Expression of p95Vav is limited exclusively to cells of hematopoietic origin, including those of the erythroid, lymphoid and myeloid lineages.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Proto-oncogene vav; VAV1; VAV
Swiss Prot
P15498
Reactivity
Human, Rat
Immunogen
Synthetic phosphopeptide derived from human VaV1 around the phosphorylation site of Tyrosine 174.
Conjugation
Unconjugated
Related Pathways
Regulation of Actin Dynamics
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 100 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-Vav1 (Y174) polyclonal antibody detects endogenous levels of Vav1 protein when phosphorylated at Tyr174.
Applications Notes
Western blot (WB) analysis of p-Vav1 (Y174) pAb at 1:500 dilution Lane1:THP-1 whole cell lysate (40ug) Lane2:HuT78 whole cell lysate (40ug) Lane3:The Spleen tissue lysate of Mouse (40ug) Lane4:The Spleen tissue lysate of Rat (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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