Syk (phospho-Y525) polyclonal antibody

Syk (spleen tyrosine kinase) is a 635 amino acid protein that contains one protein kinase domain and two SH2 domains. One of several members of the protein kinase superfamily, Syk functions as a positive effector of B cell antigen receptor (CD79) -stimulated responses, coupling CD79 with the movement of one calcium ion through one of two phospho-regulated pathways. Via its ability to influence CD79 activity and to control the movement of calicum through the cell, Syk plays an important role in a variety of cellular responses, including differentiation, phagocytosis, proliferation and B cell development. Syk expression is upregulated in T cell lymphoma, suggesting a possible role for Syk in tumorigenesis. Two isoforms of Syk, designated short and long, exist due to alternative splicing events.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of p-Syk (Y525) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:HEK293T treated with UV for 5 minutes then repair for 4 hours whole cell lysate (40ug) Lane3:HEK293T treated with UV for 5 minutes then repair for 6 hours whole cell lysate (40ug) Lane4:HEK293T treated with UV for 5 minutes then repair for 16 hours whole cell lysate (40ug) Lane5:HEK293T treated with UV for 5 minutes then repair for 24 hours whole cell lysate (40ug) Lane6:The Lung tissue lysate of Rat (40ug) Lane7:The Uterus tissue lysate of Mouse (40ug)