Stat6 (phospho-Y641) polyclonal antibody
Product Specifications
Background
Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Signal transducer and activator of transcription 6; IL-4 Stat; STAT6
Swiss Prot
P42226
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Stat6 around the phosphorylation site of Tyrosine 641.
Conjugation
Unconjugated
Related Pathways
Jak Stat : IL-6 Receptor Signaling
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 110 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-Stat6 (Y641) polyclonal antibody detects endogenous levels of Stat6 protein only when phosphorylated at Tyr641
Applications Notes
Western blot (WB) analysis of p-Stat6 (Y641) pAb at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:A2780 whole cell lysate (40ug) Lane3:SK-OVCAR3 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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