Stat4 (phospho-Y693) polyclonal antibody
Product Specifications
Background
Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Signal transducer and activator of transcription 4; Stat-4; Stat 4
Swiss Prot
Q14765
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Stat4 around the phosphorylation site of Tyrosine 693.
Conjugation
Unconjugated
Related Pathways
Jak Stat : IL-6 Receptor Signaling
Applications
WB IHC IP
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 81 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-Stat4 (Y693) polyclonal antibody detects endogenous levels of Stat4 protein only when phosphorylated at Tyr693
Applications Notes
Western blot (WB) analysis of p-Stat4 (Y693) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:MCF-7 treated with IFN-γ (100ng/ml,15 minutes) whole cell lysate (40ug) Lane3:MCF-7 treated with IFN-γ (100ng/ml,30 minutes) whole cell lysate (40ug)
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
Curated Selection
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