Stat3 (phospho-Y705) polyclonal antibody

Product Specifications

Background

Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Signal transducer and activator of transcription 3; Acute-phase response factor; STAT-3; APRF

Swiss Prot

P40763

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Stat3 around the phosphorylation site of Tyrosine 705.

Conjugation

Unconjugated

Related Pathways

Immune Cell Signaling, Jak Stat : IL-6 Receptor Signaling

Applications

WB IHC IP

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 88 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Stat3 (Y705) polyclonal antibody detects endogenous levels of Stat3 protein only when phosphorylated at Tyr705

Applications Notes

Western blot (WB) analysis of Stat3 (phospho-Y705) polyclonal antibody at 1:500 dillution Lane1:Hela whole cell lysate (40μg) Lane2:Hela treated with IFN-γ (100ng/ml, 30min) whole cell lysate (40μg) Lane3:Jurkat whole cell lysate (40μg) Lane4:Jurkat treated with H2O2 (100nM/ml, 30min) whole cell lysate (40μg)