Stat1 (phospho-Y701) polyclonal antibody

Product Specifications

Background

Signal transducer and activator of transcription that mediates signaling by interferons (IFNs) . Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84; STAT1; STAT1α; STAT1β

Swiss Prot

P42224

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Stat1 around the phosphorylation site of Tyrosine 701.

Conjugation

Unconjugated

Related Pathways

Immune Cell Signaling, Jak Stat : IL-6 Receptor Signaling, Signaling Pathways Activating p38 MAP Kinase

Applications

WB IHC IP

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 87 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Stat1 (Y701) polyclonal antibody detects endogenous levels of Stat1 protein only when phosphorylated at Tyr701.

Applications Notes

Western blot (WB) analysis of p-Stat1 (Y701) pAb at 1:500 dilution Lane1:U-87MG whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:The Embyro tissue lysate of Mouse (40ug) Lane5:Panc1 whole cell lysate (40ug)