SRF (phospho-S103) polyclonal antibody
Product Specifications
Background
Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF-binding sites are also constitutive promotor elements in many muscle-specific promotors. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk-1 and SRF accessory protein-1 (SAP-1) have DNA binding properties identical to that of p62TCF. Elk-1 and SAP-1 contain two homologous regions of which the two amino-terminal regions, the Ets domain (box A) and the B box, mediate ternary complex formation with SRF.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Serum response factor
Swiss Prot
P11831
Reactivity
Human
Immunogen
Synthetic phosphopeptide derived from human SRF around the phosphorylation site of Serine 103.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 52 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-SRF (S103) polyclonal antibody detects endogenous levels of SRF protein when phosphorylated at Ser103.
Applications Notes
Western blot (WB) analysis of SRF (phospho-S103) polyclonal antibody at 1:500 dilution Lane1:H1792 whole cell lysate (40ug) Lane2:SK-OVCAR3 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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