SMC 1 (phospho-S957) polyclonal antibody
Product Specifications
Background
The SMC (structural maintenance of chromosomes) family of proteins form heterodimeric complexes that modulate sister chromatid cohesion and chromosome condensation for mitosis. The two distinct classes of SMC protein complexes are comprised of SMC1 (also designated SB1.8) with SMC3 (also designated HCAP for human chromosome-associated protein, and Bamacan), and SMC2 (also designated hCAP-E) with SMC4 (also designated hCAP-C) . The SMC1/SMC3 complex is required for metaphase progression in mitotic cells and functions independently of the SMC2/SMC4 complex during the cell cycle. SMC1 is ubiqitiously expressed as a 150 kDa protein in various human tissues, including thymus, testis, and colon. SMC3 is expressed as a 146 kDa nuclear protein in the colon, but can also occur as a secreted proteoglycan (called Bamacan) expressed in testis and brain. Bamacan contains several glycosylation sites and is thought to play a role in basement membrane physiology.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Structural maintenance of chromosomes protein 1A; SMC protein 1A; SMC-1-alpha; SMC-1A; Sb1.8; SMC1A; DXS423E; KIAA0178; SB1.8; SMC1; SMC1L1
Swiss Prot
Q14683
Reactivity
Human
Immunogen
Synthetic phosphopeptide derived from human SMC 1 around the phosphorylation site of Serine 957.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 143 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-SMC1 (S957) polyclonal antibody detects endogenous levels of SMC1protein only when phosphorylated at Ser957
Applications Notes
Western blot (WB) analysis of p-SMC 1 (S957) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate (40ug) Lane2:SGC7901 treated with UV for 5 minutes then repair for 1 hours whole cell lysate (40ug) Lane3:SGC7901 treated with UV for 5 minutes then repair for 3 hours whole cell lysate (40ug) Lane4:SGC7901 treated with UV for 5 minutes then repair for 16 hours whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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