MEK-4/ MAP2K4 (phospho-S80) polyclonal antibody

Product Specifications

Background

The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK 5, whereas MEK-6 phosphorylates p38 and p38β. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Dual specificity mitogen-activated protein kinase kinase 4; MAP kinase kinase 4; MAPKK 4; JNK-activating kinase 1; MAPK/ERK kinase 4; MEK 4; SAPK/ERK kinase 1; SEK1; Stress-activated protein kinase kinase 1; SAPK kinase 1; SAPKK-1; SAPKK1; c-Jun N-terminal kinase kinase 1; JNKK; MAP2K4; JNKK1; MEK4; MKK4; ; PRKMK4; SEK1; SERK1; SKK1

Swiss Prot

P45985

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human MEK-4 around the phosphorylation site of Serine 80.

Conjugation

Unconjugated

Related Pathways

SAPK JNK Signaling Cascades, Signaling Pathways Activating p38 MAP KinaseMAPK Erk in Growth and Differentiation Pathway

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 44 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-MEK-4/ MAP2K4 (S80) polyclonal antibody detects endogenous levels of MEK-4 protein only when phosphorylated at Ser80.

Applications Notes

Western blot (WB) analysis of MEK-4 (phospho-S80) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate Lane2:L02 treated with EGF (0.1ng/ml, 5min) whole cell lysate Lane3:L02 treated with EGF (0.1ng/ml, 10min) whole cell lysate Lane4:L02 treated with EGF (0.1ng/ml, 15min) whole cell lysate