MEK-4/ MAP2K4 (phospho-S80) polyclonal antibody
Product Specifications
Background
The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK 5, whereas MEK-6 phosphorylates p38 and p38β. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Dual specificity mitogen-activated protein kinase kinase 4; MAP kinase kinase 4; MAPKK 4; JNK-activating kinase 1; MAPK/ERK kinase 4; MEK 4; SAPK/ERK kinase 1; SEK1; Stress-activated protein kinase kinase 1; SAPK kinase 1; SAPKK-1; SAPKK1; c-Jun N-terminal kinase kinase 1; JNKK; MAP2K4; JNKK1; MEK4; MKK4; ; PRKMK4; SEK1; SERK1; SKK1
Swiss Prot
P45985
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human MEK-4 around the phosphorylation site of Serine 80.
Conjugation
Unconjugated
Related Pathways
SAPK JNK Signaling Cascades, Signaling Pathways Activating p38 MAP KinaseMAPK Erk in Growth and Differentiation Pathway
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 44 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-MEK-4/ MAP2K4 (S80) polyclonal antibody detects endogenous levels of MEK-4 protein only when phosphorylated at Ser80.
Applications Notes
Western blot (WB) analysis of MEK-4 (phospho-S80) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate Lane2:L02 treated with EGF (0.1ng/ml, 5min) whole cell lysate Lane3:L02 treated with EGF (0.1ng/ml, 10min) whole cell lysate Lane4:L02 treated with EGF (0.1ng/ml, 15min) whole cell lysate
Host or Source
Rabbit
Available Sizes
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