Rb (phospho-S795) polyclonal antibody
Product Specifications
Background
The retinoblastoma tumor suppressor gene product, known as Rb or pRb, regulates differentiation, apoptosis and cell cycle control by coordinating the cell cycle, at G1/S, with transcriptional machinery that includes the heterodimeric E2F family. During G1, cyclin D (D1, 2, 3) -dependent kinase-mediated phosphorylation of Rb at Ser 795 marks the conversion of Rb from a transcriptionally repressive, hypophosphorylated state to an inactive, phosphorylated state, which may be sustained through mitosis by differential phosphorylation of up to 16 putative serine or threonine residues, including Ser 249/Thr 252, Thr 373, Thr 356, Ser 780, Ser 807/Ser 811 and Thr 821/Thr 826.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110; RB1
Swiss Prot
P06400
Reactivity
Human
Immunogen
Synthetic phosphopeptide derived from human Rb around the phosphorylation site of Serine 795.
Conjugation
Unconjugated
Related Pathways
Cell Cycle Control G1 S Checkpoint
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 106 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-Rb (S795) polyclonal antibody detects endogenous levels of Rb protein only when phosphorylated at Ser795
Applications Notes
Western blot (WB) analysis of p-Rb (S795) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate (40ug) Lane2:SGC7901 treated with PBS (1xPBS, PH7.4) for 3 hours whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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