Rb (phospho-S795) polyclonal antibody

Product Specifications

Background

The retinoblastoma tumor suppressor gene product, known as Rb or pRb, regulates differentiation, apoptosis and cell cycle control by coordinating the cell cycle, at G1/S, with transcriptional machinery that includes the heterodimeric E2F family. During G1, cyclin D (D1, 2, 3) -dependent kinase-mediated phosphorylation of Rb at Ser 795 marks the conversion of Rb from a transcriptionally repressive, hypophosphorylated state to an inactive, phosphorylated state, which may be sustained through mitosis by differential phosphorylation of up to 16 putative serine or threonine residues, including Ser 249/Thr 252, Thr 373, Thr 356, Ser 780, Ser 807/Ser 811 and Thr 821/Thr 826.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110; RB1

Swiss Prot

P06400

Reactivity

Human

Immunogen

Synthetic phosphopeptide derived from human Rb around the phosphorylation site of Serine 795.

Conjugation

Unconjugated

Related Pathways

Cell Cycle Control G1 S Checkpoint

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 106 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Rb (S795) polyclonal antibody detects endogenous levels of Rb protein only when phosphorylated at Ser795

Applications Notes

Western blot (WB) analysis of p-Rb (S795) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate (40ug) Lane2:SGC7901 treated with PBS (1xPBS, PH7.4) for 3 hours whole cell lysate (40ug)