PDPK1 (phospho-S241) polyclonal antibody
Product Specifications
Background
PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of hosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
3-phosphoinositide-dependent protein kinase 1; hPDK1; PDPK1; PDK1
Swiss Prot
O15530
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human PDPK1 around the phosphorylation site of Serine 241.
Conjugation
Unconjugated
Related Pathways
Phosphoinosotode Signaling, Protein Kinase C Signaling, Translational Contral elF4 and p70 S6 Kinase, Inhibition of Apoptosis, Insulin &Glucose Signaling
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 63 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-PDPK1 (S241) polyclonal antibody detects endogenous levels of PDPK1 protein when phosphorylated at Ser241.
Applications Notes
Western blot (WB) analysis of p-PDK1 (S241) pAb at 1:1000 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:HEK293T treated with EGF (100ng/ml,30 minutes) whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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