NFkB-p100 (phospho-S865) polyclonal antibody
Product Specifications
Background
The NFκB transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino-terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFκB. It is synthesized as a protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFκB subunit, in this case a protein designated p52 (previously referred to as p49) . In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light; polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10; Lyt10; NFKB2; LYT10
Swiss Prot
Q00653
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human NFkB-p100 around the phosphorylation site of Serine 865.
Conjugation
Unconjugated
Related Pathways
NF-kB Signaling, STING Signaling, Inhibition of Apoptosis
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 100, 120 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-NFkB-p100 (S865) polyclonal antibody pAb detects endogenous levels of NFkB-p100 protein only when phosphorylated at Ser865.
Applications Notes
Western blot (WB) analysis of NFkB-p100 (phospho-S865) polyclonal antibody at 1:500 dilution Lane1:NIH-3T3 whole cell lysate (40ug) Lane2:NIH-3T3 (PMA, 60ng/ml, 5min) whole cell lysate (40ug) Lane3:NIH-3T3 (PMA, 60ng/ml, 15min) whole cell lysate (40ug) Lane4:NIH-3T3 (PMA, 60ng/ml, 30min) whole cell lysate (40ug) Lane5:NIH-3T3 (EGF, 0.1ng/ml, 30min) whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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