Myc (phospho-S62) polyclonal antibody

Product Specifications

Background

C-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64; MYC; BHLHE39

Swiss Prot

P01106

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Myc around the phosphorylation site of Serine 62.

Conjugation

Unconjugated

Related Pathways

Warburg Effect, Signaling Pathways Activating p38 MAP KinaseCell Cycle Control G1 S Checkpoint, Glutamine Metabolism

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 49, 57, 65 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Myc (S62) polyclonal antibody detects endogenous levels of proto-oncogene c-Myc protein only when phosphorylated at Ser62.

Applications Notes

Western blot (WB) analysis of p-c-Myc (S62) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with UV Lane2:NIH-3T3 cell lysate treated with Forskolin Lane3:PC12 cell lysate treated with UV