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MEF-2 (phospho-T312) polyclonal antibody

Product Specifications

Background

The myocyte enhancer factor-2 (MEF-2) family of transcription factors associated with co-repessors or co-activators to regulate development and function of T cells, neuronal cells and muscle cells. Four family members arise from alternatively spliced transcripts, termed MEF-2A, -2B, -2C and -2D. These members bind as homo- and heterodimers to the MEF-2 site in the promoter region of affected genes. Differential regulation in the expression of the four transcripts implies functional distinction for each during embryogenesis and development. The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Myocyte-specific enhancer factor 2A; Serum response factor-like protein 1; MEF2A; MEF2

Swiss Prot

Q02078

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human MEF-2 around the phosphorylation site of Threonine 312.

Conjugation

Unconjugated

Related Pathways

Signaling Pathways Activating p38 MAP Kinase, Examples of Crosstalk Between Post-translational Modifications

Applications

IHC IP

Dilution

IHC: 1:50~1:200 IP: 1:10~1:100

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 55 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-MEF-2 (T312) polyclonal antibody detects endogenous levels of MEF-2 protein only when phosphorylated at Thr312

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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