Histone H3 (phospho-S10) polyclonal antibody

Product Specifications

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Histone H3.1; HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.2; Histone H3/m; Histone H3/o; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.3; H3F3A; H3.3A; H3F3; H3F3B; H3.3B

Swiss Prot

P68431/Q71DI3/P84243

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Histone H3 around the phosphorylation site of Serine 10.

Conjugation

Unconjugated

Related Pathways

Histone Lysine Methylation Pathway, Examples of Crosstalk Between Post-translational Modifications, DNA Methylation

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 17 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Histone H3 (S10) polyclonal antibody detects endogenous levels of Histone H3 protein only when phosphorylated at Ser10.

Applications Notes

Western blot (WB) analysis of p-Histone H3 (S10) pAb at 1:1000 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (10ug) Lane3:PC3 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug)