GSK3α/β (phospho-Y279/216) polyclonal antibody

Product Specifications

Background

Glycogen synthase kinase-3 α and β (GSK-3α, β) are serine/threonine kinases that regulate metabolic enzymes and transcription factors, which are responsible for coordinating processes such as glycogen synthesis and cell adhesion. GSK-3β activity is also required for nuclear activity of Rel dimers, which mediate an anti-apoptotic response to TNFα in mice. GSK-3 catalytic kinase activity is controlled through differential phosphorylation of serine/threonine residues, which have an inhibitory effect, and tyrosine residues, which have an activating effect. Growth factor stimulation of mammalian cells expressing GSK-3α and GSK-3β induces phosphorylation of Ser 21 and Ser 9, respectively through a phosphatidylinositol 3-kinase (PI 3-kinase) -protein kinase B (PKB) dependent pathway, thereby enhancing proliferative signals. Additionally, GSK-3 physically associates with cAMP-dependent protein kinase A (PKA), which phosphorylates Ser 21 of GSK-3α or Ser 9 of GSK-3β and inactivates both forms

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3A

Swiss Prot

P49840/P49841

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human GSK3α around the phosphorylation site of Tyrosine 279.

Conjugation

Unconjugated

Related Pathways

PI3K AKT signaling, Hedgehog Signaling, Hypoxia Signaling, Translational Contral elF4 and p70 S6 Kinase, Translational Contral Regulation of elF2, Regulation of Microtubule Dynamics, Inhibition of Apoptosis, Insulin &Glucose Signaling, Cell Cycle Control G1 S Checkpoint

Applications

WB IHC

Dilution

WB: 1:500~1:2000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 46,51 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-GSK3α/β (Y279/216) polyclonal antibody detects endogenous levels of GSK3α/β protein only when phosphorylated at Tyr279/216

Applications Notes

Western blot (WB) analysis of GSK3α/β (phospho-Y279/216) polyclonal antibody at 1:2000 dilution Lane1:L02 whole cell lysate Lane2:L02 treated with PBS (1xPBS, PH7.4) for 30min whole cell lysate Lane3:L02 treated with PBS (1xPBS, PH7.4) for 60min whole cell lysate Lane4:L02 treated with PBS (1xPBS, PH7.4) for 120min whole cell lysate Lane5:L02 treated with PBS (1xPBS, PH7.4) for 180min whole cell lysate Lane6:L02 treated with PBS (1xPBS, PH7.4) for 240min whole cell lysate Lane7:C6 whole cell lysate