GSK3α (phospho-S21) polyclonal antibody

Product Specifications

Background

Glycogen synthase kinase-3 α and β (GSK-3α, β) are serine/threonine kinases that regulate metabolic enzymes and transcription factors, which are responsible for coordinating processes such as glycogen synthesis and cell adhesion. GSK-3β activity is also required for nuclear activity of Rel dimers, which mediate an anti-apoptotic response to TNFα in mice. GSK-3 catalytic kinase activity is controlled through differential phosphorylation of serine/threonine residues, which have an inhibitory effect, and tyrosine residues, which have an activating effect. Growth factor stimulation of mammalian cells expressing GSK-3α and GSK-3β induces phosphorylation of Ser 21 and Ser 9, respectively through a phosphatidylinositol 3-kinase (PI 3-kinase) -protein kinase B (PKB) dependent pathway, thereby enhancing proliferative signals. Additionally, GSK-3 physically associates with cAMP-dependent protein kinase A (PKA), which phosphorylates Ser 21 of GSK-3α or Ser 9 of GSK-3β and inactivates both forms

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3A

Swiss Prot

P49840

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human GSK3α around the phosphorylation site of Serine 21.

Conjugation

Unconjugated

Related Pathways

Translational Contral elF4 and p70 S6 Kinase, Regulation of Microtubule Dynamics

Applications

WB IHC IP

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 51 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-GSK3α (S21) polyclonal antibody detects endogenous levels of GSK3α protein only when phosphorylated at Ser21 .

Applications Notes

Western blot (WB) analysis of p-GSK3α (S21) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with UV Lane2:Mouse liver tissue lysate Lane3:Rat liver tissue lysate Lane4:PC12 cell lysate treated with H2O2