GR (phospho-S226) polyclonal antibody

Product Specifications

Background

The glucocorticoid receptor (GR) is a ubiquitously expressed transcription factor that mediates the effects of glucocorticoids. The most abundant isoform is GR α. GR induces or represses the expression of genes in response to glucocorticoids, mediating such processes as apoptosis, cell growth and differentiation. A significant class of genes suppressed by GR is controlled by the transcription factor AP-1. GR has also been shown to be the limiting factor in the induction of gene expression by glucocorticoids. It has been revealed that GR forms a complex with HSP 90, rendering the non-ligand bound receptor transcriptionally inactive. More importantly, mutant GRs lacking the signaling domain remain constitutively active.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Glucocorticoid receptor; GR; Nuclear receptor subfamily 3 group C member 1; NR3C1; GRL

Swiss Prot

P04150

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human GR around the phosphorylation site of Serine 226.

Conjugation

Unconjugated

Related Pathways

Nuclear Receptor Signaling, Nuclear Receptor Signaling

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 90 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-GR (S226) polyclonal antibody detects endogenous levels of GR protein only when phosphorylated at Ser226

Applications Notes

Western blot (WB) analysis of p-NR3C1 (S226/234/246) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate (40ug) Lane2:SGC7901 treated with UV for 5 minutes then repair for 1 hour whole cell lysate (40ug) Lane3:SGC7901 treated with UV for 5 minutes then repair for 3 hours whole cell lysate (40ug)