GR (phospho-S226) polyclonal antibody
Product Specifications
Background
The glucocorticoid receptor (GR) is a ubiquitously expressed transcription factor that mediates the effects of glucocorticoids. The most abundant isoform is GR α. GR induces or represses the expression of genes in response to glucocorticoids, mediating such processes as apoptosis, cell growth and differentiation. A significant class of genes suppressed by GR is controlled by the transcription factor AP-1. GR has also been shown to be the limiting factor in the induction of gene expression by glucocorticoids. It has been revealed that GR forms a complex with HSP 90, rendering the non-ligand bound receptor transcriptionally inactive. More importantly, mutant GRs lacking the signaling domain remain constitutively active.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Glucocorticoid receptor; GR; Nuclear receptor subfamily 3 group C member 1; NR3C1; GRL
Swiss Prot
P04150
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human GR around the phosphorylation site of Serine 226.
Conjugation
Unconjugated
Related Pathways
Nuclear Receptor Signaling, Nuclear Receptor Signaling
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 90 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-GR (S226) polyclonal antibody detects endogenous levels of GR protein only when phosphorylated at Ser226
Applications Notes
Western blot (WB) analysis of p-NR3C1 (S226/234/246) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate (40ug) Lane2:SGC7901 treated with UV for 5 minutes then repair for 1 hour whole cell lysate (40ug) Lane3:SGC7901 treated with UV for 5 minutes then repair for 3 hours whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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