Filamin 1 (phospho-S2152) polyclonal antibody

Product Specifications

Background

Caldesmon, Filamin 1, nebulin and villin are differentially expressed and regulated Actin binding proteins. Both muscular (CDh) and non-muscular (CDl) forms of caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and Myosin. CDh is expressed predominantly on thin filaments in smooth muscle, whereas CDl is widely expressed in nonmuscle tissues and cells. Filamin 1, which is ubiquitously expressed and exists as a homodimer, functions to crosslink Actin to filaments. Nebulin is a large filamentous protein specific to muscle tissue that may function as a ruler for filament length. Several isoforms of nebulin, ranging from 700 to 900 kDa, are produced by alternative exon usage. Villin is Ca2+-regulated and is the major structural component of the brush border of absorptive cells.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

FilaminA; Filamin A; Filamin-A; FLN-A; Actin-binding protein 280; ABP-280; Alpha-filamin; Endothelial actin-binding protein; Filamin-1; Filamin1; Non-muscle filamin; FLNA; FLN; FLN1

Swiss Prot

P21333

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Filamin 1 around the phosphorylation site of Serine 2152.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 280 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Filamin 1 (S2152) polyclonal antibody detects endogenous levels of Filamin 1 protein when phosphorylated at Ser2152.

Applications Notes

Western blot (WB) analysis of Filamin 1 (phospho-S2152) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:A549 whole cell lysate (20ug) Lane3:The Lung tissue lysate of Rat (20ug) Lane4:AML-12 whole cell lysate (40ug) Lane5:CT26 whole cell lysate (40ug) Lane6:SKOVCAR3 whole cell lysate (40ug) Lane7:Panc1 whole cell lysate (40ug)