EGFR (phospho-T654) polyclonal antibody
Product Specifications
Background
EGFR (Epidermal growth factor receptor, ErbB-1) is a receptor tyrosine kinase (RTK) that is one of four members of the EGFR/ErbB family of receptor tyrosine kinases. EGFR plays a key role in the regulation of essential normal cellular processes and in the pathophysiology of hyperproliferative diseases such as cancer. Activation of the EGFR signaling pathway has been linked with increased cell proliferation, angiogenesis, metastasis and decreased apoptosis. Phosphorylation of residue Thr654 (removal of the signal peptide) during PKC activation prevents binding of calmodulin.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Epidermal growth factor receptor; EC=2.7.10.1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; ERBB; ERBB1; HER1; EGFR
Swiss Prot
P00533
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human EGFR around the phosphorylation site of Threonine 654.
Conjugation
Unconjugated
Related Pathways
ErbB HER Signaling
Applications
IHC
Dilution
IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 175 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-EGFR (T654) polyclonal antibody detects endogenous levels of EGFR protein only when phosphorylated at Thr654 (removal of the signal peptide) .
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
