What is the catalog number for EGFR (phospho-S1046) polyclonal antibody?

The catalog number for EGFR (phospho-S1046) polyclonal antibody is BS4061.

What sizes are available for EGFR (phospho-S1046) polyclonal antibody?

EGFR (phospho-S1046) polyclonal antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does EGFR (phospho-S1046) polyclonal antibody come in?

EGFR (phospho-S1046) polyclonal antibody is available in 2 different pack sizes.

Is EGFR (phospho-S1046) polyclonal antibody in stock?

EGFR (phospho-S1046) polyclonal antibody is currently made to order / not held in immediate stock. Contact us for lead time.

EGFR (phospho-S1046) polyclonal antibody

Product Specifications

Background

EGFR (Epidermal growth factor receptor, ErbB-1) is a receptor tyrosine kinase (RTK) that is one of four members of the EGFR/ErbB family of receptor tyrosine kinases. EGFR plays a key role in the regulation of essential normal cellular processes and in the pathophysiology of hyperproliferative diseases such as cancer. Activation of the EGFR signaling pathway has been linked with increased cell proliferation, angiogenesis, metastasis and decreased apoptosis. Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Epidermal growth factor receptor; EC=2.7.10.1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; ERBB; ERBB1; HER1; EGFR

Swiss Prot

P00533

Reactivity

Human

Immunogen

Synthetic phosphopeptide derived from human EGFR around the phosphorylation site of Serine 1046.

Conjugation

Unconjugated

Related Pathways

ErbB HER Signaling

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 175 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

EGFR (phospho-S1046) polyclonal antibody detects endogenous levels of EGFR protein only when phosphorylated at serine 1046 (removal of the signal peptide) .

Applications Notes

Western blot (WB) analysis of EGFR (phospho-S1046) polyclonal antibody at 1:500 dilution Lane1:The Lung tissue lysate of Mouse (40ug) Lane2:The Lung tissue lysate of Rat (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:SKOVCAR3 whole cell lysate (20ug) Lane5:HCC827 whole cell lysate (10ug)